Persévérer avec Christ/ Persevering with Christ
Par Charles Tchapdeu /By Charles Tchapdeu
Interpreté en Anglais par Tristan Kabongo
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Isoptin 80 generico ) in a 1 × 10 −8 dilution of the serum was used as standard control. All the samples were used within 10 min of the start incubation in same well, as shown by standard curves to the left. Blood was collected from an antecubital vein of the upper arm participant for 24 h and analyzed by liquid chromatography with ultraviolet detection (LC-UV) and high-performance liquid chromatography–mass spectrometry (high-performance chromatography–tandem mass spectrometry; Agilent) in the GCMS. After sample collection, plasma was separated from any blood by passing it through a 3 × 150 μm mesh cell strainer. All the plasma samples from this study were then subjected to standard chemical analyses. Plasma insulin levels were analysed in plasma using LC-C18 mass spectrometry. All samples were collected after a 15 min centrifugation within the first hour post-exercise (within 2 h of awakening), and the plasma insulin concentration was measured using immunoassays with the use of and ELISA kit (Ventana Instruments, Ventura, California, USA) which was used in this study [ 14 ]. An insulin dose of 2 μg/ml and 10 mM glucose in plasma were used for all the tests. Plasma insulin levels were expressed as nmol/ml in ng/ml the same way as insulin concentration using standard curve generated with the method of Kahl, et al; [ 15 ] at the beginning of 24-h period. Body mass was assessed using a calibrated scale (Seca, São Paulo, Brazil). The measurement was made before assessment of physical exercise intensity to allow the measurement of difference in body mass between the groups. All the physical performance tests (push-ups, leg presses, sit-ups, and jumping rope) measured during the pre-exercise period were performed by the same individual in position using the same apparatus within a time-point of the 4th hour recovery period within the 2-h protocol. Each exercise was followed by 4 min of rest before the next test. During testing, 2 groups did not differ in the number of repetitions each exercise. Before the exercise on day prior to the study, participants were asked to take a warm-up and drink caffeinated 30 min prior to any exercise they planned perform on the day of experiment. All exercises performed within the experimental protocol were chosen based on the criteria provided by current guidelines of the scientific community on body weight (weight loss or maintenance) and energy expenditure body composition (weight retention or weight maintenance) after training with resistance exercise [ 12 ]. The programmes contained a set of exercises that were selected based to produce maximum muscle activation and were done in a progressive manner without interruption. This set of exercises was chosen because the high effectiveness (i.e. in exercise program) of resistance training that allows the adaptation of muscle size, strength and hypertrophy, hyperactivation muscle activation [ 12, 13 ], in accordance with the results described by Fussenegger and colleagues [ 14 ]. The protocols for strength and metabolic performance tests were the same for both groups. A group of 22 normal weight (25.4 ± 1.3 kg, 70.8 4.7%BMI; body mass index 25–29 kg/m 2; age 26.3 ± 1.2 years) female volunteers voluntarily recruited and were provided the same food and nutritional requirements in order to maintain the required study conditions. Each subject was recruited, selected and randomly assigned into 2 groups. Before their participation to the study, each of participants signed an informed consent form and underwent medical examinations. After signing the consent form, participants were divided into two groups by using a random number generator. They were not asked to change the clothes and underwear for duration of the study. In order to prevent influence of possible drug use, both groups received the same oral contraceptives Oxybutynin buy online uk and were kept in the same group. They were also evaluated with a body fat analysis before starting the study. In the last week of control period before beginning the intervention, in order to minimize the metabolic consequences of weight loss (e.g. changes in metabolic rate), the control group received a 2-h overnight-fasting before the intervention [ 23 ]. We chose to start the intervention immediately in order to avoid possible changes the hormonal regulation that might increase the levels of body weight or fat. Prior to beginning the study, participants gave their written informed consent and had completed the screening questionnaire to Brazilian Society for Testing and Clinical Research, Brazilian Ministry of Health, Research Ethics Committee, the committee of Federal University Paraná and the of Goiás. At 2–6 weeks, subjects were randomly assigned into the two groups by using same random number generator.